The Oral Glucose Tolerance Test ( Ogtt ) - 1333 Words

Introduction The oral glucose tolerance test (OGTT) is used to measure the response of the body to glucose. More specifically, the test has been used for decades in the clinical as well as experimental environment as an ‘index of the efficiency of the mechanisms regulating the concentration of glucose in the blood’ (Baird et al., 1959). It has proved to be an important tool in the diagnosis of diabetes mellitus, and is commonly used in the screening of gestational diabetes in women. Diabetes mellitus is characterized by a hindered ability to remove blood glucose during carbohydrate intake due to either a decreased amount of secreted insulin, as seen in Type 1 diabetics, or a lack of response to insulin, as in Type 2 diabetics. (Mayo†¦show more content†¦Diluted serum samples, previously collected from three patients during the tolerance test, were then dispensed according to the time they were taken. The patients had been asked to fast overnight, and the following morning we re requested to ingest 75 g of glucose administered via solution, after which blood samples were taken from the brachial vein at 0, 30, 60, 90, 120, 150 and 180 minutes for measurement of blood glucose levels (Kim et al., 2016). Following this, 200 ÃŽ ¼l Glucose Reagent (containing buffer, hexokinase, glucose 6-phosphate dehydrogenase, NAD+, ATP and Mg2+) was added within a period of 90s to each of the samples of glucose standard as well as serum samples. All mixtures were finally incubated at room temperature for 15 minutes, and the serum glucose level of fasting for the three subjects retrieved using a colorimetric method. (Kim et al., 2016) Results The standard curve (Fig. 1) generated using the resulting absorbance of the glucose standard displays the variation in absorbance at 340nm with the amount (nmol) of standard present in the sample. A linear trend is clearly visible, with a calculated gradient of 0.0156. Different patterns were observed for the variation in glucose concentration of serum samples taken from the three patients at equal time intervals over a period of 180 minutesShow MoreRelatedRisk Factors : Description Of Gestational Diabetes Mellitus Screening1265 Words   |  6 Pages(American Diabetes Association, 2016). It occurs in approximately 7% of pregnant women across the world and 6% of pregnancies in the United States (Caissutti Berghella, 2017). GDM occurs when there is insulin resistance leading to glucose intolerance or high levels of glucose in the blood. According to the American College of Obstetrics and Gynecologists, most cases of GDM resolve once the pregnant female delivers the child (2016). 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There are many tests that are used for the screening of type 2 diabetesRead MoreDiabetes Is The Most Common Form Of Diabetes1723 Words   |  7 Pagesregularly scheduled foot exams, eye exams, and A1C tests. To be effective, cooperation and compliance are required for the successful implementation of a tertiary strategy. The plans success is linked on the patient taking personal responsibility for the disease and adherence to the treatment regimen. Screening Programs In order for preventive programs to be effective people who have the disease must be identified and staged. There are many tests that are used for the screening of type 2 diabetesRead MoreGestational Diabetes Mellitus : An Overview1241 Words   |  5 Pagesactions are not being utilized. This occurrence leads to hyperglycemia; an issue in which the amount of glucose in the blood is relatively high. Gestational Diabetes Mellitus falls under the category of DM. GDM also consist of a form of insulin resistance throughout the pregnancy of the woman. If a woman is to be high-risk to have diabetes at the first prenatal visit; they should receive a test to be screened for diabetes. The majority of woman get diagnosed (GDM) throughout the second and third trimesterRead MoreGestational Diabetes Mellitus : An Overview1241 Words   |  5 Pagesactions are not being utilized. This occurrence leads to hyperglycemia; an issue in which the amount of glucose in the blood is relatively high. Gest ational Diabetes Mellitus falls under the category of DM. GDM also consist of a form of insulin resistance throughout the pregnancy of the woman. If a woman is to be high-risk to have diabetes at the first prenatal visit; they should receive a test to be screened for diabetes. The majority of woman get diagnosed (GDM) throughout the second and third trimesterRead MoreDiabetes Mellitus, Often Overlooked as Not Serious When It Actually Is575 Words   |  3 Pagesmore normal life than was possible 200 years ago. There are a number of tests that can be performed to accurately check glucose levels in the blood to determine whether someone has diabetes. The A1C test measures the blood glucose average for the two to three months prior to the test. Many people choose to have the A1C test done because unlike all of the other diabetes testing the patient does not have to fast or consume a glucose drink. Diabetes is diagnosed when an A1C comes back with levels greater